The evaluation of the antimicrobial capacity of various compounds and materials for their potential application in different areas is essential. Studies on biofilms are particularly relevant nowadays, as their formation represents a considerable risk in sectors such as medicine and food industry, which underlines the need of effective antimicrobial compounds to inhibit or reduce their formation. On the other hand, it is crucial to analyse the effect of different compounds on biofilm formation and disassembling when they are used in biotechnological processes, as in these cases the aim is to preserve the integrity of biofilms, so that they can contribute positively to these processes. In both contexts, the evaluation is performed by in vitro assays, which offers several advantages, such as the use of simple biological models that avoid ethical considerations, high reproducibility, shorter analysis times and reduced costs, among others.